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KMID : 0379120080360020148
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Korean Journal of Mycology
2008 Volume.36 No. 2 p.148 ~ p.152
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Selection of Ectomycorrhizal Isolates of Tricholoma matsutake and T. magnivelare for Inoculation on Seedlings of Pinus densiflora In Vitro
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Ka Kang-Hyeon
Park Hyun
Hur Tae-Chul
Bak Won-Chull
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Abstract
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We inoculated hypal suspension of Tricholoma matsutake and T. magnivelare were examined on Pinus densiflora seedlings grown in a granite soil substrate with 1/2 PDMP (12 g/l potato dextrose broth, 1.5 g/l malt extract, and 0.5 g/l peptone) medium. Four months after inoculation, the pine seedlings were examined for infection rate, matsutake aroma, and Hartig-net formation. The roots of pine seedling formed ectomycorrhizal roots in the 9 isolates from 12 isolates of T. matsutake and T. magnivelare. However, the seedlings showed different ectomycorrhizae forming rates among the 9 isolates. While matsutake aroma was confirmed from the ectomycorrhizal seedlings, the pine seedling contaminated by bacteria or fungi did not form matsutake ectomycorrhizae with sickening smell. Thus, the aroma was chosen as a good way for the verification of mycorrhizal infection. At the early stage, the mycorrhizal roots showed unramified and branched types without root hair. They also showed thin mantle layers, Hartig-nets, and turned into black color at later stage. Among the examined strains, that of Yecheon isolated in 1995 showed the best infection rate, which indicated that we need to pay attention to the selection of isolates for better result.
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KEYWORD
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Eectomycorrhizal formation, Pinus densiflora, Tricholoma magnivelare, Tricholoma matsutake
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